Phenotypic and molecular detection of esbl-producing genes tem-1 and shv-1 in uropathogenic escherichia coli isolates

Background: Spread of pathogenic strains carrying genes Extended-spectrum beta-lactamases [ESBL] is one of the major health concerns in the world. These genes caused due to the inefficient beta-lactam antibiotics in the treatment of infectious diseases. The purpose of this study was to determine the prevalence of TEM-1 and SHV-1 beta-lactamase genes in uropathogenic E. coif isolates from the city of Khorramabad

Methods: A total of 100 E. coli isolates were collected from patients with urinary tract infections in clinical laboratories from city of Khorramabad. Originality isolates were confirmed by biochemical tests. ESBLs positives isolates were determined by using a combined disk based instruction the Clinical and Laboratory Standards Institute [CLSI] and then all the positive isolates were studied by PCR assay for the presence of genes TEM-1 and SHV-1

Results: Thirty one [31%] out of 100 E. coif isolates were ESBL positive based on the results of combined disc tests. PCR analysis using the specific primers revealed that 18 isolates [58.06%] contained p-lactamases genes encoding TEM, and while none of isolates did not carry SHV gene

Conclusion: The relatively high prevalence of resistance genes to third generation of cephalosporins among the E. coif isolates was found which indicates a challenge in successful treatment with current antibiotics

[Prevalence of Shiga toxin-producing genes in Escherichia coli isolated from patients with urinary tract infections in Khorramabad]

Background and Objectives: Shiga toxins [verotoxin] is one of the most important bacterial toxin produced by the Escherichia coli [O157: H7 and nonO157: H7], and is responsible for various diseases in humans and animals. Hemolytic uremic syndrome [HUS] is serious human diseases that occur because of these toxins. There is no comprehensive information in this regard, here will studied the prevalence of these genes among the E- coli isolated from urine samples in Khorramabad

Material and Method: The study is based on 146 Escherichia coli isolates of Patients with symptoms of urinary tract infection referred to clinical laboratories in Khorramabad during 90-1389. We used we identified the isolates by biochemical tests .The Multiplex PCR Multiplex PCR method were used to presence of genes

Results: A total of 117 [13/80%] of the women and 29 [86/19] of the men and none of them carried both stx1and stx2

Conclusion: Shiga toxin-producing E. coli isn't responsible for urinary tract infection in KhorramabadAlthough the frequency of these genes in urine samples is low, but evaluation of the other human and animal samples in different parts of the country is essential

Molecular characterization of a Salmonella typhimurium isolate from Caspian pony

Typhoid disease or salmonellosis is a common sickness in horses. In several epidemiological studies in hospitalized horses, several serotypes of Salmonella often are predominant in nosocomial infections. Transportation, overcrowding, dehydration, oral antimicrobial therapy and infections are the risk factors which may activate latent or subclinical salmonellosis. In this study, the occurrence of typhoid due to Salmonella serogroup B was considered in a Caspian ponies flock kept in a husbandry center of ponies around Tehran. During transportation of 19 ponies, two pregnant ponies aborted and four cases died because of acute septicemia. Pathological and bacteriological follow up showed salmonellosis. A multiplex poly-merase chain reaction [m-PCR] assay was used for detection and identification of Salmonella to confirm pathological and bacteriological studies. Salmonella typhimurium was isolated from bone marrow, mesenteric lymph nodes, liver and intestinal contents of died pony. Salmonella was not isolated from stools of other ponies. Pulsed Field Gel Electrophoresis [PFGE] and antibiotic susceptibility test were also performed. PFGE pattern was similar to the other collected isolates which have existed since more than 30 years ago in Iran. Because of importance of salmonellosis in ponies, using rapid methods are recommended to confirm the presence of Salmonella. Results showed that m-PCR permit to evaluate samples more rapidly than other methods and also can detect multiple genes simultaneously like virulence factors which declare virulence of the isolates and have surveillance significances